Hydrogenase activity in Rhodopseudomonas capsulata: relationship with nitrogenase activity
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چکیده
منابع مشابه
Regulation of Nitrogenase Activity in Rhodopseudomonas capsulata
Regulation o f nitrogenase activity in Rhodopseudomonas capsulata AD 2 differs in several respects from other Rhodospirillaceae: 1) Nitrogenase activity in this strain grown under severe N-starvation was fully derepressed, but in contrast to other Rhodospirillaceae, it was inhibited by am m onia in vivo. 2) Nitrogenase in extracts o f glutamate grown cells was fully active and not further stimu...
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A system for genetic exchange in Rhodopseudomonas capsulata has been discovered. Each genetic marker thus far examined can be transferred, and many strains of Rps. capsulata can participate in genetic exchange. The mechanism of gene transfer seems unlike that of any previously described bacterial system, since genes can be transferred by cell-free filtrates, but the vector is resistant to deoxy...
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The isolation and study of glycerol-utilizing mutants of Rhodopseudomonas capsulata indicated that the wild-type organism has genes capable of coding for the catabolism of glycerol but is unable to express them. Furthermore, the genetic lesion in the original glycerol-utilizing mutant, L1, occurred very close to these genes.
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Hydrogenase activity of root nodules in the symbiotic association between Pisum sativum L. and Rhizobium leguminosarum was determined by incubating unexcised nodules with tritiated H(2) and measuring tissue HTO. Hydrogenase activity saturated at 0.50 millimolar H(2) and was not inhibited by the presence of 0.10 atmosphere C(2)H(2), which prevented H(2) evolution from nitrogenase. Total H(2) pro...
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A Rhodopseudomonas capsulata nifH::lacZ gene fusion was used to isolate constitutive mutants of R. capsulata, unable to repress nif gene transcription anaerobically with every fixed-nitrogen source tested. When these nifc strains were grown aerobically, nif gene transcription was repressed. These results indicate that the regulation of nif gene transcription by fixed nitrogen is different from ...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1980
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.144.1.141-148.1980